The study is a human study for healthy volunteers who for three weeks replace their usual diet with a ketogenic diet. Metabolic, microbiotic, neurobiological and clinical parameters are measured before and after the study. This means that a whole-body perspective is taken into account when it comes to health, but also the mental part as well as how the mentally status linked to the dietary change. Many of the body's processes are governed by what we eat. What has recently been shown is that a ketogenic diet can reverse both obesity and type 2 diabetes and also affect cancer. A diet consisting of ketones, broken down fatty acids, has been found to have a number of positive benefits over traditional carbohydrate-rich diets. The microbiota (intestinal flora) has also been shown to play a crucial role in well-being and health status.
Blood samples will be analyzed focusing on the following areas, inflammation, metabolism, blood fats and stress.
The effect of the diet on the gut microbiota will be examined in each trial participant, analysis will be performed before and after the study. The composition of the bacterial flora in the intestine is determined by sequencing bacterial DNA from stool samples. The presence of more than 300 bacterial species is analyzed using 54 different DNA probes. By identifying the composition of intestinal flora before and after the dietary intervention, we can study how the microbiota is altered by a ketogenic diet. In addition, we can study whether alteration of microbiota can be linked to metabolic effects of dietary intervention by comparing microbiota in study participants who respond and do not respond to the intervention. This knowledge can also provide a basis for identifying prognostic fecal biomarkers that show which individuals are likely to respond positively to a ketogenic diet.
The effect of the diet on both stress but also the impact on perceived well-being and KaSam (sense of coherance) will be investigated. KaSam is a view of health and illness as a multidimensional phenomenon, health is not an on/off condition. KaSam is an instrument / questionnaire for measuring life attitudes that can increase resistance to stress and in a way be health-promoting. Health surveys SF-36, gastro-, gastrointestinal, PID-5, and psychological well-being surveys will also be used. The forms will be used before and after the dietary intervention, thus measuring the effect of the dietary intervention purely neurobiologically and also psychologically / perceptually.
The following clinical parameters will be measured before and after the study: Heart Rate Variability (HRV), blood pressure, fat percentage, weight, blood ketones, blood glucose, waist measurements, stub measurements, and a blood sample for biochemical analysis. Ultrasound examination of the heart and vessels measures possible cardiovascular changes.